Acquisition of Two Evosep One Instruments

The primary focus at the Novo Nordisk Foundation Center for Protein Research is basic research within protein-related areas, including protein signaling and pathways, protein structure, disease systems biology and particularly technological developments within mass spectrometry-based proteomics.

The procurement of two Evosep One HPLCs is an upgrade/addition to existing high-performance LC-MS setups at the proteomics platform at Novo Nordisk Foundation Center for Protein Resesarch (CPR), University of Copenhagen. The Evosep systems will be part of the advanced analytical toolset employed at CPR for basic and translational protein research. Specifically, the instruments will be used for high-throughput analysis of clinically-relevant proteins and proteomes by separating peptides in line with electrospray tandem mass spectrometry.

No other supplier is able to provide analytical LC equipment with similar specifications for high-throughput, low-flow and sensitive analysis of complex peptide samples as an integrated LC-MS solution. The unique features of the relevant equipment are:

Use of disposable trap columns:

• reduces carry-over in e.g. complex HeLa digests to < 0.05 % of TIC;

• reduces cycle time (<5min per sample).

Partial elution from trap columns.

• leaves impurities on the disposable traps, thereby greatly increasing column life.

Gradient offset focusing (where the organic %B-gradient is lowered concurrently with the elution and pre-separation of peptides in a pre-formed gradient);

• allows shorter columns/improves resolving power on analytical column.

Fast trap-elution and gradient formation at 30μl/min.

Use of a long and narrow storage loop as hand-over between the low-pressure, high-flow gradient preparation and the high-pressure, low-flow gradient delivery.

Optimized high-throughput capabilities:

• up to 300 samples/day @ 1-2 μl/min flow rate;

• up to 93 % MS utilization rate;

• whisper flow technology with gradient flow of 100 nl/min at 40 samples/day for ultra-sensitive applications, such as single cell proteomics.

Only Evosep Bioscience can provide an integrated solution that allows high-throughput of complex peptide samples (up to 300 samples/day) and ensures high utilization rate of the MS instrumentation (up to 93 %)