Fakta om udbudet
Udbyder
University of Copenhagen
Vindere
(31.01.2022)
Evosep A/S
Buchwaldsgade 35, 3.
5000 Odense C
Acquisition of an Evosep One instrument
University of Copenhagen
Voluntary ex ante transparency notice
Supplies
Section I: Contracting authority/entity
Postal address: Nørregade 10
Town: Copenhagen K
NUTS code: DK01 Hovedstaden
Postal code: 1165
Country: Denmark
Contact person: Mahmut Yüksel
E-mail: Fgn253@ku.dk
Internet address(es):
Main address: www.KU.dk
Section II: Object
Acquisition of an Evosep One instrument
Proteomics Research Infrastructure (PRI) is a state of-the-art mass spectrometry (MS)-based proteomics facility. Founded on 1 July 2020, the new facility provides coordinated, proprietary client access to forerunner proteomic technology, exceptional technical assistance, and streamlined bioinformatic data analysis for the local research community. PRI is funded by the Novo Nordisk Foundation (NNF) and linked to the NNF Center for Protein Research at the University of Copenhagen.
The procurement is a purchase of an additional Evosep One instrument to the Proteomics Research Infrastructure (PRI) at the University of Copenhagen. The procurement will be used to support research and development across the University of Copenhagen. As the support will be based upon existing technologies at CPR, the PRI will have to be equipped with same methodologies and technologies as available at CPR in order to be able to provide same type of analytical support.
Section IV: Procedure
- The works, supplies or services can be provided only by a particular economic operator for the following reason:
- absence of competition for technical reasons
- Additional deliveries by the original supplier ordered under the strict conditions stated in the directive
No other supplier is able to provide analytical LC equipment with similar specifications for high-throughput, low-flow and sensitive analysis of complex peptide samples as an integrated LC-MS solution. The unique features of the relevant equipment are:
Use of disposable trap columns:
• Reduces carry-over in e.g. complex HeLa digests to < 0.05% of TIC.
• Reduces cycle time (<5min per sample).
Partial elution from trap columns.
• Leaves impurities on the disposable traps, thereby greatly increasing column life.
Gradient offset focusing (where the organic %B-gradient is lowered concurrently with the elution and pre-separation of peptides in a pre-formed gradient)
• Allows shorter columns / improves resolving power on analytical column.
Fast trap-elution and gradient formation at 30μl/min.
Use of a long and narrow storage loop as hand-over between the low-pressure, high-flow gradient preparation and the high-pressure, low-flow gradient delivery.
Optimized high-throughput capabilities:
• Up to 300 samples/day @ 1-2 μl/min flow rate
• Up to 93% MS utilization rate
The services offered by the PRI will be based upon the technological developments established at CPR. Thus in order to provide this, the equipment purchased for the PRI needs to be similar to the one available at CPR.
No other supplier can provide an integrated LC-MS solution that allows similar throughput. The purchaser needs a system that allow for analysis of several hundreds of clinically relevant peptide mixtures per day per MS instrument, and the requirement for a system able to integrative and automatically handle sample injection and chromatographic peptide separation in a short time-frame is of the utmost importance. In order to analyze up to 300 samples per day by MS specifically requires that loading of sample and chromatographic separation of peptide mixtures by reverse-phase chromatography are handled within 5 minutes per individual sample (total run time). This in turn ensures high utilization of MS instrumentation, and only hereby enables high-throughput analyses.
The intended procurement complements the 3 existing Evosep One instruments in our facility. The new instrument must be identical to the other instruments to ensure internal comparability, data reproducibility as well as alignment with existing systems.
Section V: Award of contract/concession
Postal address: Buchwaldsgade 35, 3.
Town: Odense C
NUTS code: DK031 Fyn
Postal code: 5000
Country: Denmark
Telephone: +45 26332322
Section VI: Complementary information
Postal address: Nævnenes hus, toldboden 2
Town: Viborg
Postal code: 8800
Country: Denmark
E-mail: NH@naevneneshus.dk
Telephone: +45 72405600
Internet address: www.KLFU.dk