Fakta om udbudet
Udbyder
University of Copenhagen
Vindere
(16.05.2022)
Phi Optics, Inc.
1800 S. Oak Street, Suite 106,
IL 61820 Champaign
Acquisition of a Gradient Light Interference Microscopy module(CellVista GLIM Pro)
University of Copenhagen
Voluntary ex ante transparency notice
Supplies
Section I: Contracting authority/entity
Postal address: Nørregade 10
Town: Copenhagen K
NUTS code: DK01 Hovedstaden
Postal code: 1165
Country: Denmark
Contact person: Mahmut Yüksel
E-mail: fgn253@ku.dk
Internet address(es):
Main address: www.KU.dk
Section II: Object
Acquisition of a Gradient Light Interference Microscopy module(CellVista GLIM Pro)
The Center for Advanced Bioimaging (CAB) Denmark is a core facility center at the University of Copenhagen, aiming to provide researchers with advanced, state-of-the-art imaging equipment, as well as consulting in data acquisition and analysis.
The center contributes to a great range of basic as well as applied research projects, both via our own and our network’s expertise and by enabling researchers to use the high-end equipment available at the center.
Imaging of intact plant tissues if often limited by the specimen’s thickness and optical density. In addition, air spaces in intact plants lead to light scattering, further degrading the obtainable image from deep optical sections. Gradient Light Interference Microscopy (GLIM) is a unique technique which can be added to existing Differential Interference Contrast (DIC) microscopes, as present at CAB, allowing for quantitative, high-contrast phase imaging in thick specimens, including 3D-reconstructions. Additionally, it is a label-free alternative to fluorescence-based methods.
Section IV: Procedure
- The works, supplies or services can be provided only by a particular economic operator for the following reason:
- absence of competition for technical reasons
The system will be sued to directly monitor cell shapes inside of plant tissues, which is almost impossible to do with other types of light microscopy. The benefits are that the samples do not need to be fixed and can be directly monitored during development.
This can for example be addressing how leaf cell architecture develop, or how roots produce lateral roots in details not previously possible.
There are no products available from other manufacturers incorporating equivalent technology (see pt. 11). In comparison to other techniques that allow deep imaging, as for example multi-photon fluorescence microscopy, the GLIM module has unique advantages:
• Making use of existing, high quality optical instruments at CAB, the GLIM module is a rather cost-effective extension of the capabilities of these instruments.
• It can be moved between instruments, as long as the optical prerequisites are met by the target microscope. This means for example, that both inverted and upright microscopes at CAB can benefit from the purchase.
• Using light of low power density compared to laser illumination allows for imaging without phototoxicity.
• Unlike fluorescence-based methods, phase imaging requires no labelling.
Section V: Award of contract/concession
Postal address: 1800 S. Oak Street, Suite 106,
Town: Champaign
NUTS code: US United States
Postal code: IL 61820
Country: United States
E-mail: chirites@phioptics.com
Section VI: Complementary information
Postal address: Nævnenes hus, toldboden 2
Town: Viborg
Postal code: 8800
Country: Denmark
E-mail: NH@naevneneshus.dk
Telephone: +45 72405600
Internet address: www.KLFU.dk