Official name: University of Copenhagen
National registration number: 29979812
Postal address: Nørregade 10
Town: Copenhagen
NUTS code: DK011 Byen København
Postal code: 1165
Country: Denmark
Contact person: Mathilde Kruse
E-mail: mak@adm.ku.dk
Telephone: +45 23840131
Internet address(es):
Main address: www.ku.dk

Body governed by public law

Education

Acquisition of a STELLARIS 8 focus on DIVE/CRS

38510000 Microscopes

Supplies

Acquisition of a STELLARIS 8 focus on DIVE/CRS for the implementation of intravital microscopy as a collaboration between the LEO Foundation Skin Immunology Research Center (SIC) and the Core Facility for

Integrated Microscopy (CFIM)

This contract is divided into lots: no

Value excluding VAT: 9 459 528.00 DKK

NUTS code: DK01 Hovedstaden

The LEO Foundation Skin Immunology Research Center aims at understanding, preventing and treating skin diseases for the benefit of the individual patient. For this, the researchers at the center need access to a multiphoton microscope to perform intravital imaging on human skin transplants and several mice models. After a thorough screening and testing of the commercially available systems, the Leica STELLARIS 8 focus on DIVE/CRS was identified as the only possible microscope for the service. The intravital services will be offered open access to other research groups in collaboration with the Core Facility for Integrated Microscopy and the Department of Experimental Medicine at SUND.

Options: no

The procurement is related to a project and/or programme financed by European Union funds: no

Negotiated procedure without prior publication

Explanation:

Our research application requires 4D deep imaging of skin tissue with a sensitive, flexible and user-friendly system.

STELLARIS 8 focus on DIVE/CRS is a true confocal point scanning system with prism-based spectral detection design with computer controlled adjustable bandwidth for all fluorescence channels and offers enhanced detection efficiency. The extended detection range up to 850 nm allows the application and separation of an extended range of spectrally overlapping fluorophores. This will allow us to label up to five cell types/tissue structures simultaneously.

LIGHTNING allows for optimal extraction of image details and maximum resolution, thus expanding the imaging portfolio both in the classical range and beyond the diffraction limit. Features a tandem scanning system to provide highest axial resolution with galvanometric scanner, switchable between resonant and non-resonant mode. This will allow us to obtain high resolution 3D information on skin structural changes.

Equipped with Power HyD S detector, new standard for detection. All-round detector, high performance throughout the spectrum, two times increased photon detection efficiency in the blue-green range compared to standard multialkaline photomultiplier. Leica Si-based technology is compatible with TauSense in combination with the white light laser. This is very important to be able to detect cells expressing low levels of fluorescent proteins deep inside the tissue.

Multiphoton extension STELLARIS 8 DIVE provides imaging in 1mm depth or beyond. Variable beam expander and beamcatcher ensure stable performance and adaption to sample requirements. 4Tune is the only spectrally tunable non-descanned detector and provides spectral flexibilty through the visible spectrum with up to 4 spectral channels simultaneously and unlimited 1 | 10 sequentially. Equipped with Power HyD NDD, 4Tune enables TauSense with multiphoton excitation. Tausense with allow us to image several fluorescent proteins deep inside the tissue flexibly and fast.

The Leica STELLARIS 8 FALCON system provides fast FLIM (at confocal speed) with any imaging workflow. Quick and easy operation is ensured by the full integration within LAS X. STELLARIS 8 FALCON includes FLIM analysis tools, such as exponential fitting, FLIM phasor analysis, pattern fitting and FLIM-FRET analyzer. STELLARIS 8 FALCON allows to quantify fast cellular processes, like molecular interaction or molecular environment changes.

The procurement is covered by the Government Procurement Agreement: yes

13/09/2022

The contract has been awarded to a group of economic operators: no

Official name: Triolab
Postal address: Vallensbækvej 35
Town: Brøndby
NUTS code: DK012 Københavns omegn
Postal code: 2605
Country: Denmark
Telephone: +45 43960012

The contractor/concessionaire will be an SME: no

Total value of the contract/lot/concession: 9 459 528.00 DKK

Patents:

• the German patent DE 102 53 108 B4 relate to the HyD S detector;

• the patents US 10,073,034 B2, CN 103105383 B, JP 6220510 B2, are in force in U.S.A, China and Japan, respectively, and the European Patent EP 2 592 413 B1 is in force in Germany, France, UK, Italy, the Netherlands, Switzerland. These patents are related to the TauSense functionalities;

• the American patent US 10,775,599 B2 is granted and in force, the German utility model DE 20 2016 008 334 U1 is registered and in force, and the European patent with the publication number EP 3 308 210 B1 is granted and in force in Germany, France and UK. This utility model and the patents relate to the 4Tune detector (DIVE);

• the patents CN 103105383 B, JP 6220510 B2, US 10,073,034 B2 are in force in China, Japan and U.S.A. and in France, respectively, and the European patent and EP 2 592 413 B1 is in force in Germany, UK and in France. These patents are related to the fluorescence Lifetime Imaging capabilities (Falcon, FAst Lifetime CONtrast).

Official name: Danish Public Contracts Appeals Board
Postal address: Nævnenes Hus, Toldboden 2
Town: Viborg
Postal code: 8800
Country: Denmark
E-mail: NH@naevneneshus.dk
Telephone: +45 72405600
Internet address: www.KLFU.dk

05/10/2022