Fakta om udbudet
Udbyder
University of Copenhagen
Vindere
(15.11.2022)
Bruker Nordic AB
Torshamnsgatan 44
SE-164 40 Kista
Acquisition of a timsTOF-fleX-MALDI-2
University of Copenhagen
Voluntary ex ante transparency notice
Supplies
Section I: Contracting authority/entity
Postal address: Nørregade 10
Town: Copenhagen K
NUTS code: DK01 Hovedstaden
Postal code: 1165
Country: Denmark
Contact person: Mahmut Yüksel
E-mail: fgn253@ku.dk
Internet address(es):
Main address: www.KU.dk
Section II: Object
Acquisition of a timsTOF-fleX-MALDI-2
The Department of Plant and Environmental Sciences at University of Copenhagen carries out research across all aspects of plant science, from the molecular level to the field. Several research groups work with plant metabolism and the chemistry of plant-microbe interactions, and the department hosts several platforms for metabolomics and imaging. The purchase of the timsTOF fleX-MALDI-2 is funded by the Novo Nordisk Foundation and will enable the establishment of a facility for mass spectrometry imaging for plant science.
The timsTOF fleX-MALDI-2 system with microGRID comes with both MS (mass spectrometry) and tims (trapped ion mobility spectrometry) detectors as well as a post-ionization laser (MALDI-2) for enhanced ionization and sensitivity, and in addition the capacity for imaging at a lateral resolution of 5 µm in square pixels to cover the entire sample. The instrument will be used for analyses of metabolites in plants and plant microbe interactions. Plants are much more metabolically complex than animals and it is paramount that instruments used for plant analyses have very high sensitivity and identification power, which will be provided by respectively MALDI-2 and the combination of accurate mass (qTOF-MS) and trapped ion mobility analyses.
Section IV: Procedure
- The works, supplies or services can be provided only by a particular economic operator for the following reason:
- absence of competition for technical reasons
In our work with imaging plant tissues, we require minimal pixel size (<10 µm) to reach single cell resolution, high identification power (qTOF-MS + tims) to identify and differentiate isobaric compounds in extremely complex mixtures and high sensitivity (MALDI-2) for detection of lowly abundant compounds and at high lateral resolution. The timsTOF fleX-MALDI-2 system with microGRID is currently the only system that combines all these features in a single instrument. We have tested the MALDI-2 function of the instrument (Murphy et al., 2022, Plant Physiology) to find ionization features typical of MALDI-2 in plant samples. Ion mobility has proven to enable separation of true isomoers and even chiral compounds, and the timsTOF-fleX MALDI-2 system is furthermore a combination instrument, with both ESI and MALDI ionization sources, that allows for ion mobility separation. Finally, the established analytical facility will have a wide range of users and a high throughput which will be supported by the Intellislides and SCiLS autopilot feature that allows automated analyses.
Patents Covering Unique Features:
- TIMS cell (BIL 031/08), Title: “Apparatus and method for parallel flow ion mobility spectrometry combined with mass spectrometry”
Issued: US7838826B1; US 8288717B2
- Temporal Zoom, IMEX (BDAL 293/11), Title: “Spectrum Acquisition Modes For Ion Mobility Spectrometers Using Trapped Ions”
Issued: US8766176B2; GB2490387B
Pending: DE102012004398A1
- Spatial Zoom (BRE 399/15, BRE 404/15), Title: “Spatial zoom mode for accumulative trapped ion mobility spectrometry”
Issued: US9304106B1; US9546980B1
Pending: EP3054475A1; EP3165913A1; US20170125234A1; CN105869980A; CN107039231A
- Parallel Accumulation TIMS (BRE 398/15), Title: “Trapping ion mobility spectrometer with parallel accumulation”
Issued: US9683964B2
Pending: EP3054473A1; CN105869983A
Section V: Award of contract/concession
Postal address: Torshamnsgatan 44
Town: Kista
NUTS code: SE Sverige
Postal code: SE-164 40
Country: Sweden
Section VI: Complementary information
Postal address: Nævnenes hus, tolboden 2
Town: Viborg
Postal code: 8800
Country: Denmark
E-mail: NH@naevneneshus.dk
Telephone: +45 72405600
Internet address: www.KLFU.dk