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Q Exactive HF with Vanquish Flex.

A Thermo Scientific Q Extractive HF with Vanquish Flex UHPLC system.

Due to the complexity of crude oil, full characterisation of its components requires a combination of multiple complimentary instrumental techniques. Currently, our laboratory has advanced gas chromatography and mass spectrometry facilities for analyse volatile/semi-volatile and low to medium molecular weight compounds. However, we lack the capability for identification of polar and high molecular weight constituents. Therefore, we wish to acquire a mass spectrometer (MS) coupled to a high-pressure liquid chromatography system.

Due to the complexity of crude oil, full characterization of its components requires a combination of multiple complimentary instrumental techniques. Currently, our laboratory has a requirement for a high-resolution MS system for petroleum analysis. Whereas mass resolution allows the separation of compounds with a near identical molecular weight, mass accuracy allows determination of molecular formula. For successful determination of molecular structure, ultra-high mass resolution and accuracy are a pre-requisite when analyzing complex mixtures. A common isobaric motif in petroleomics is C3 and SH4 (Δm = 3.4 mDa), which requires a resolution of >100k for separation at m/z 400 (assuming equal peak intensity). At m/z 800, the required resolution is >230k. The Q Extractive series of instruments uniquely meets these requirements on the market.

To accommodate the varying ionization potential of different compound classes, the instrument should have a swappable ionization source (with options for ESI, APPI, APCI) and operate in positive and negative mode. Further, to analyze species not separable by chromatography the instrument should have the option of direct infusion and a possibility of future upgrades with ambient ionization sources.

The Vanquish flex is the natural choice for coupling to an Orbitrap MS as the same software suite can control both components. Additionally, we currently have a Dionex Ultimate 3000 SD system, which is the previous model, for semi-preparative fractionation, which means that we will have the added benefit from being familiar with the system and having interchangeable spare parts and consumables.

Internet address:https://erhvervsstyrelsen.dk/klagenaevnet-for-udbud

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