Fakta om udbudet
Udbyder
University of Copenhagen
Vindere
(02.10.2018)
Bruker Nordic AB
Vallagatan 5
SE-170 67 Solna
Acquisition of a TimsTOF Pro MS System
University of Copenhagen
Voluntary ex ante transparency notice
Supplies
Directive 2014/24/EU
Section I: Contracting authority/entity
Nørregade 10
Copenhagen
1017
Denmark
Contact person: Jens Hoejer Larsen
E-mail: jens.hoejer.larsen@adm.ku.dk
NUTS code: DK011
Internet address(es):
Main address: www.oko-okonomi@adm.ku.dk
Section II: Object
Acquisition of a TimsTOF Pro MS System
The acquisition concerns the timsTOF Pro MS system, which comes with a dual-TIMS (trapped ion mobility spectrometry) analyzer optimized for high-speed shotgun proteomics. The University of Copenhagen intends to use this instrument for clinical research, where we will measure proteins from various patient samples.
The timsTOF Pro system comes with a dual-TIMS (trapped ion mobility spectrometry) analyzer optimized for high-speed shotgun proteomics. Its unique geometry allows incoming ions to be accumulated in parallel in the first section, and for ions to be released dependent on their mobility from the second section of the dual-TIMS analyzer. This results in nearly 100 % duty cycle, making this Parallel Accumulation Serial Fragmentation Technique (PASEF) perfectly suited for nanoflow LC-MS analysis of enzymatically digested complex protein mixtures. Measurements in both, MS and PASEF mode. Our sister laboratory at MaxPlanck institute for biochemistry, Munich has already tested this technology (Meier et al, Journal of Proteome Research 2015, Meier et al Bioarchive, 2018).
The timsTOF Pro system provides unique technology for identification of peptides which is highly relevant for our research. The timsTOF from Bruker Nordic AB is the only instrument in the market, which has both TIMS technology allowing PASEF acquisition method.
The contracting institution is the Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, which intends to use this instrument for clinical research, where we will measure proteins from various patient samples.
Section IV: Procedure
- The works, supplies or services can be provided only by a particular economic operator for the following reason:
- absence of competition for technical reasons
The timsTOF Pro comes with the Trapped ion mobility which allows Parallel Accumulation Serial Fragmentation (PASEF) acquisition method to provide extremely high speed and sensitivity to reach new depths in shotgun proteomics. Our sister laboratory at MaxPlanck institute for biochemistry, Munich has already tested this technology (Meier et al Bioarchive, 2018). We demonstrated that more than 800 000 fragmentation spectra in standard 120 min LC runs are easily achievable, which can be used for near exhaustive precursor selection in complex mixtures or re-sequencing weak precursors. Online PASEF achieves a remarkable sensitivity with more than 2 900 proteins identified in 30 min runs of only 10ng HeLa digest. This technology is highly relevant for clinical research, which is our main focus. Bruker Nordic AB is the only provider of this instrument, which provides unique technology for identification of peptides and metabolites.
Section V: Award of contract/concession
Vallagatan 5
Solna
SE-170 67
Sweden
Telephone: +46 086552500
E-mail: sales.ms.nordic@bruker.com
Fax: +46 086552599
NUTS code: SE110
Internet address: https://www.bruker.com
Section VI: Complementary information
Nævnenes Hus, Toldboden 2
Viborg
8800
Denmark
Telephone: +45 72405600
E-mail: klfu@naevneneshus.dk
Internet address: https://erhvervsstyrelsen.dk/klagenaevnet-for-udbud