Internet address(es):

Main address: https://www.ku.dk

Aquisistion of a LMD7 Leica Unique Laser Micro Dissection Microscope

A fully automated laser micro dissection microscope that will enable us to automatically import masks from previously detected and segmented cells of interest. The system is moving the laser, not the sample, which is extremely important for our setup. This laser will cut along the masked area of interest. It will be equipped with a variety of collecting devices, like strips of 8 wells, 96 well plate, 96 micro tube plate and the system is collecting the dissected samples via gravity, which is crucial for our workflow. Those dissected samples will then be analyzed by Mass Spectrometry.

A fully automated laser micro dissection microscope will enable experiments that are currently not possible with the equipment on site. It will enable us to automatically import masks from previously detected and segmented cells of interest. The system is moving the laser, not the sample, which is extremely important for our setup. This laser will cut along the masked area of interest. It will be equipped with a variety of collecting devices, like strips of 8 wells, 96 well plate, 96 micro tube plate and the system is collecting the dissected samples via gravity, which is crucial for our workflow. Those dissected samples will then be analyzed by Mass Spectrometry.

University of Copenhagen — Faculty of Health and Sciences comprises a wide range of expertise and skills within its research programs spanning disease systems biology, proteomics, high throughput protein production and characterization, chemical biology, disease biology, and protein therapeutics.

The center contributes to the progress of translational research within medicine and provides fundamental insights which can be used to promote drug discovery and development.

Explanation:

— Leica LMD7 moves the laser, not the sample,

— cuts directly, real-time with “Move and Cut”,

— specimen collection by gravity. This prevents any possible contamination and stress to the sample during the removal phase,

— collects samples into strips of 8 wells, 96 well plate, 96 micro tube plate,

— LMD7 is the only one to use a solid-state UV laser pulse with a wavelength of 349 nm, whereby one can adjust laser power and Aperture directly during the laser cut.

DE10018255C2 laser aperture stop allows adjustment of the laser beam diameter and such the width of the cutting line without influencing the visual image.

US7035004B2 and EP1276586B1 prism device and its control unit which directs the laser beam through the objective and allows moving of the laser beam in the visual field of view while the stage is stopped.

DE10018251C2 and US6907798B2 LMD specific stage with receiving unit allows to move the receiving unit and to bring only the opening of the selected receiving element into the correct catching position for catching the dissectate.

DE10057292C2 and EP1207392B1 LMD PCR-Tube holder device with movable mounting elements for PCR-Tubes as collecting containers for dissectates.

DE102005028062C5 and US8535876B2 LMD cutting mode for fast cutting of dissectates.

EP1719998B1 and US7485878B2 allow active laser cutting in fluorescence modus.

US9217694B2 automated detection of dissectates and automated definition of cutting lines without the need of hand-input of marking lines.

Internet address: https://www.erhvervsstyrelsen.dk/klagenaevnet-for-udbud