Internet address(es):

Main address: https://permalink.mercell.com/109296864.aspx

Address of the buyer profile: http://www.sdu.dk/

Stimulated Emission Depletion Microscope

Purchase of a stimulated emission depletion microscope for research purposes at The University of Southern Denmark (SDU).

General description:

3D Stimulated emission depletion microscope optimized for super resolution live cell and tissue imagine. The instrument will be part of a core facility and will be used super resolution imaging of a range of applications from fixed samples to live cell and tissue imaging. Key aspects of the instrument will be, lower light dosage, multiphoton excitation STED, STED fluorescence correlation microscopy (FCS) and adaptive optics for to compensate for sample-induced aberrations.

The University of Southern Denmark (SDU).

The following features are requested to facilitate optimal performances for our applications:

— live cell STED imaging: a key research requirement is optimization for live-cell imaging. Abberior Instruments currently is, to the best of our knowledge, the sole supplier of a live cell package that consists of a variety of unique technologies: DyMIN, RESCue, Minfield, and easy3D-STED. Each of these methods offers complementary advantages when it comes to performing diverse experiments in high-class research. DyMIN is an exclusive technology that reduces the laser irradiation dose by orders of magnitude through intelligent adaption of the laser intensity as it is scanned across the sample. This way, bleaching can be reduced by typically an order of magnitude, allowing recording of about ten times as many images of the same resolution or recording of an image at 50 % better resolution than before. With this, our STED-microscopes are able to attain sub-30nm (2D), respectively sub-80nm (3D) optical resolutions routinely, up to our knowledge the best on the market by far. Abberior Instruments' corresponding publication can be found under J. Heine, et al., Adaptive-illumination STED nanoscopy, PNAS (2017), 201708304. RESCue: method and device for high resolution imaging of a sample structure labelled with fluorescent markers. EP 3 159 676 B1, WO 2017/067859 A2.

— adaptive optics: to the best of our knowledge, Abberior Instruments is the sole supplier offering adaptive optics in combination with STED microscopy. With the adaptive optics module using a deformable mirror, additionally to the STED laser beam all laser beams can be modulated to correct for refractive index mismatches, to enhance deep-focusing capability and to improve the signal-to-noise ratio, allowing for high-quality deep tissue imaging. The adaptive optics package can be combined with the two-photon laser, opening up a variety of research possibilities for deep tissue imaging,

— multiphoton excitation STED: Incorporation of a Mai Tai DeepSee Ti:Sapphire laser for multiphoton excitation and multiphoton STED,

— Easy3D-module: the easy3D-module, which endows the user with 2 important capabilities. First, the immersion media of the objective lens is not pre-determined in our system. Usually, only oil-based objective lenses can be used, in particular with 3D-STED. In contrast, Abberior's easy3D-technology enables the use of arbitrary types of objective lenses (air, water, silicon oil, glycerol, oil, etc.) together with 3D-STED and thereby allows for focusing depths essentially up to the working distance of the objective lens, usually several 100 μm. This feature is especially important when it comes to deep tissue imaging. Second, our easy3D-module can be used to pre-compensate certain aberrations that occur when imaging through layers varying in density, such as tissue. This further enhances deep-focusing capabilities, reduces photobleaching and cell damage, and improves the attainable resolution and signal-to-noise ratio. (3D-STED with arbitrary objective lenses: EP 3 200 002 A1, JP 2017-138591 A, US 2017/0212340 A1) and (3D-STED with a single beam and aberration control: ‘Device for separately modulating the wave fronts of 2 components of a light beam and microscope comprising the device’. DE 10 2014 113 716 A1DE 20 2015 105 046 U1 (Utility Patent), US 9,632,297 B1).

Public Procurement Act Section 80, litre 3 and 4 — Only one supplier:

The stimulated emission depletion microscope can only be supplied by one particular economic operator due to technical reasons. There is an objective and proportionate reason for the establishment of specifications which result in there being only one supplier.

The possibility that there are other potential suppliers who could deliver the same has been objectively excluded by market consultations.

Internet address: http://www.klfu.dk

Pursuant to the Danish act on complaints board for public procurement (act No 492 of 12.5.2010 with subsequent amendments — available at www.retsinformation.dk), the following time limits for filing a complaint apply: Complaints regarding a candidate not being pre-qualified must be filed with The complaints board for public procurement within 20 calender days starting the day after the Contracting Authority has sent notification to the candidates involed, cf. § 171 (2) of the Danish Act on Public Procurement (act No 1564 of 15.12.2015 — available at www.retsinformation.dk) of § 7 (1) of the Danish Act on the complaints board for public procurement, provided that the notification includes a short account of the relevant reasons for the decision. Other complaints must be filed with the complaints board for public procurement within:

1) 45 calendar days after the Contracting Authority has published a contract award notice in the Official Journal of the European Union (with effect from the day following the publication date);

2) 30 calendar days starting the day after the Contracting Authority has informed the tenderers in question, that the Contracting Authority has entered into a contract based on a Framework Agreement through reopening of competition or a dynamic purchasing system, provided that the notification includes a short account of the relevant reasons for the decision;

3) 6 months starting the day after the Contracting Authority has sent notification to the candidates/tenderers involved that the Contracting Authority has entered into the Framework Agreement, cf. § 2 (2) or § 171 (4) of the Act on Public Procurement, provided that the notification included a short account of the relevant reasons for the decision;

4) 20 calendar days starting the day after the Contracting Authority has published a notice concerning his decision to uphold the contract, cf. § 185 (2) of the Act on Public Procurement. The complainant must inform the Contracting Authority of the complaint in writing at the latest simultaneously with the lodge of the complaint to the complaints board for public procurement stating whether the complaint has been lodged in the stand-still period, cf. § 6 (4) of the Act on The complaints board for public procurement. If the complaint has not been lodged in the stand-still period, the complainant must also state whether it is requested that the appeal is granted delaying effect, cf. § 12 (1) of the said Act. The email of the complaints board for public procurement is stated in Section VI.4.1. The complaints board for public procurement's own guidance note concerning complaints is available at the internet address stated in Section VI.4.1

Internet address: http://www.kfst.dk