Official name: University of Copenhagen
Postal address: Nørregade 10
Town: Copenhagen K
NUTS code: DK01 Hovedstaden
Postal code: 1165
Country: Denmark
Contact person: Mahmut Yüksel
E-mail: FGN253@ku.dk
Internet address(es):
Main address: www.KU.dk

Body governed by public law

Education

Acquisition of a Oxford Nanopore Promethion 24

38900000 Miscellaneous evaluation or testing instruments

Supplies

The Promethion will purchased from Oxford Nanopore Technologies (ONT), and involves a sequencing machine and regular shipments of chemistry kits used for sequencing. The unique machine is able to generate single molecule sequencing reads spanning hundreds of kilobases in real-time. The machine will be used by BRIC researchers to perform nucleic acid sequencing of biological material. The equipment will be placed in the sequencing core at BRIC.

This contract is divided into lots: no

Value excluding VAT: 1 460 000.00 DKK

NUTS code: DK01 Hovedstaden

ONT was founded in 2005 to develop a disruptive, electronic, single molecule sensing system based on nanopore science. The company has a unique nanopore platform that uses ion current changes across the pore to identify nucleotide composition of long nucleic acid molecules.

Options: no

The procurement is related to a project and/or programme financed by European Union funds: no

Negotiated procedure without prior publication

Explanation:

The ONT sequencing machine will be able to provide unprecedented resolution of genetic and epigenetic alterations in both patient-derived tissue and various biological models, ranging from bacteria and viruses to complex organisms, such as higher plants and mammals. Several important projects require the usage of ONT including:

Genome phasing and resolving complex SVs, mRNA isoforms, RNA degradation, RNA splicing isoforms in mouse models, RNA ribosome modifications, DNA methylation modifications.

Oxford Nanopore has a broad patent portfolio, through in-house development and licensing agreements with third parties. Oxford Nanopore has an intellectual property portfolio of more than 650 issued patents and patent applications, in over 200 patent families. These cover all aspects of nanopore sensing including fundamental patents for nanopore sensing, and patents relating to DNA-sequencing.

List of patents: https://patents.justia.com/assignee/oxford-nanopore-technologies-limited

The following themes are included in Oxford Nanopore's patent portfolio:

• DNA base identification using a biological nanopore;

• addition of adapters to nanopores for sensing;

• modified nanopores for sensing;

• use of molecular motors in combination with nanopores;

• localising polymerases to a surface, including pore-bound localisation;

• use of solid-state nanopores for detecting labelled ssDNA and dsDNA;

• use of solid-state devices to control movement of polymers;

• detection and positional measurement of probes on a DNA strand as the strand passes through a nanoscale detector;

• methods of fabricating solid-state nanopores including multi-layered devices;

• the use of functionalised solid-state nanopores for molecular characterisation, including graphene, tunnelling currents and nanotubes;

• time-based multiplexed nanopore measurements on a single chip, including the incorporation of 96 well plate;

• use of voltage to control DNA under feedback;

• measurement of DNA interacting with a limited volume, such as a polymerase on a surface;

• array chip for parallel sensing from multiple channels;

• sequencing by means of detection of products of enzymatic action on DNA/RNA using a nanopore;

• the use of multiple 'nodes' to analyse samples in a federated/clustered manner to reduce the time to result, and improve operating efficiencies;

• methods and algorithms for nanopore signal analysis;

• Nanopore arrays/nanopore array structures;

• Direct RNA sequencing;

• protein characterisation using nanopores;

• chip/array architecture;

• methods of sensitivity enhancement.

The procurement is covered by the Government Procurement Agreement: yes

30/10/2020

The contract has been awarded to a group of economic operators: no

Official name: Oxford Nanopore Technologies
Postal address: Edmund Cartwright House 4 Robert Robinson Avenue
Town: Oxford
NUTS code: UK UNITED KINGDOM
Postal code: OX4 4GA
Country: United Kingdom
E-mail: Jakob.Oertvig@nanoporetech.com
Telephone: +44 8450347900
Internet address: www.nanoporetech.com

The contractor/concessionaire will be an SME: no

Total value of the contract/lot/concession: 1 460 000.00 DKK

Official name: Danish Public Contracts Appeal Board
Postal address: Nævnenes hus, toldboden 2
Town: Viborg
Postal code: 8800
Country: Denmark
E-mail: NH@naevneneshus.dk
Telephone: +45 72405600
Internet address: www.KLFU.dk

08/01/2021