Fakta om udbudet
Udbyder
University of Copenhagen
Vindere
(24.01.2020)
Carl Zeiss A/S
Bregnerødvej 133
3460 Birkerød
Acquisition of a Zeiss LSM980 with Airyscan2 and Axiozoom with Apotome2 to the Core Facility for Integrated Microscopy
University of Copenhagen
Voluntary ex ante transparency notice
Supplies
Directive 2014/24/EU
Section I: Contracting authority/entity
Postal address: Nørregade 10
Town: Copenhagen K
NUTS code: DK01
Postal code: 1165
Country: Denmark
Contact person: Mahmut Yüksel
E-mail: Fgn253@ku.dk
Telephone: +45 20571357
Internet address(es):
Main address: www.KU.dk
Section II: Object
Acquisition of a Zeiss LSM980 with Airyscan2 and Axiozoom with Apotome2 to the Core Facility for Integrated Microscopy
An additional inverted fluorescence confocal microscope is needed in order to manage the huge demand of users at CFIM for imaging their samples. It will enable us to image cells and tissues under natural conditions with an environmental control. It will be equipped with the Airyscan detector, which allows us to image with lower light intensity and higher resolution, which is essential for many of the projects/applications.
CFIM also has a need for a new stereo/zoom microscope which can acquire both overview images as well as more detailed images for both Brightfield and Fluorescence. The zoom microscope will be equipped with the ApoTome, which allows for optical sectioning.
An additional inverted fluorescence confocal microscope is needed in order to manage the huge demand of users at CFIM for imaging their samples. It will enable us to image cells and tissues under natural conditions with an environmental control. It will be equipped with the Airyscan detector, which allows us to image with lower light intensity and higher resolution, which is essential for many of the projects/applications.
CFIM also has a need for a new stereo/zoom microscope which can acquire both overview images as well as more detailed images for both Brightfield and Fluorescence. The zoom microscope will be equipped with the ApoTome, which allows for optical sectioning.
Section IV: Procedure
- The works, supplies or services can be provided only by a particular economic operator for the following reason:
- absence of competition for technical reasons
With the ZEN Connect software the AxioZoom.V16 and the LSM 980 can be used for correlative microscopy to quickly acquire an overview image on the zoom microscope. The overview image can then be used for navigation on the confocal microscope. This will increase the productivity on the confocal microscope.
The LSM 980 with Airyscan 2 is the only laser scanning confocal microscope that allows us to acquire images with lower laser light intensity and higher resolution. It is a point scanning confocal with super resolution based on pixel reassignment detection technology. This will give an isotropic increase in resolution down 120 nm lateral and 350 nm axial. The new multiplex mode provides greatly enhanced imaging speed (8 x faster) than confocal imaging while providing better resolution and signal to noise. This allows gentle imaging at low laser power for high-speed processes and also productive imaging of large fields of view in combination with tile experiments with enhanced image quality.
US8705172
Method for enhancing the resolution of a microscope during the detection of an illuminated specimen and a microscope for carrying out the method, wherein in a first position, an illumination pattern is generated on the specimen, the resolution of which is preferably within the range of the attainable optical resolution of the microscope or higher, wherein a relative movement, preferably perpendicular to the direction of illumination, from a first into at least 1 second position of the illumination pattern on the specimen is generated at least once between the detection and the illumination pattern with a step width smaller than the resolution limit of the microscope and detection and storage of the detection signals take place both in the first and in the second position.
EP2825908
High-resolution scanning microscopy
Summary excerpt: a microscope for high-resolution scanning microscopy of a sample, with a spatial resolution which, taking account of the imaging scale, is at least twice as high as a full width at half maximum of the diffraction-limited individual image, to have: a detector array.
Claimed in EP2825908.
The AxioZoom.V16 with the ApoTome.2 represents the most powerful combination of a large 16:1 zoom range with the highest basic NA 0.25 in the market. The ApoTome.2 is a device for structured illumination to acquire multichannel 3D fluorescence images of sections and whole animals. This provides images with increased resolution and increased spatial resolution. With this system, users at CFIM can acquire both macroscopic images of large samples as well as 3D images of e.g. cleared samples.
Section V: Award of contract/concession
Postal address: Bregnerødvej 133
Town: Birkerød
NUTS code: DK0
Postal code: 3460
Country: Denmark
E-mail: info.microscopy.dk@zeiss.com
Telephone: +45 70157015
Internet address: www.zeiss.dk
Section VI: Complementary information
Postal address: Nævnenes hus, Tolboden 2
Town: Viborg
Postal code: 8800
Country: Denmark
E-mail: NH@naevneneshus.dk
Telephone: +45 72405600
Internet address: www.KLFU.dk